Proposition de stage pour le Master de Physique Médicale – Spécialité Imagerie.
Description:
Recent research has demonstrated promising anti-tumor results with novel exposure to non- thermal plasma (NTP). Its specific action on tumors is not well understood, but it has been shown that NTP reactive oxygen species (ROS) can modify endothelial cell proliferation or enhance apoptosis. Noninvasive multiparametric ultrasonic (US) evaluation of tumors can reveal underlying changes in tumor microstructure and microvascularization thus enabling noninvasive monitoring to better understand the therapeutic action of sonosensitization and NTP.
US data will be acquired in an ectopic murine model prior to and following exposure to NTP and sham therapy. Real-time evaluation of microvascular flow based on tracer detection models for flow will be made with contrast-enhanced functional US. Effective scattering size and acoustic attenuation and elastographic maps related to tissue microstructure will be assessed using spectral and statistical analysis of radiofrequency-US signals. Parametric, 3D maps (~ 260 µm resolution) will be calculated from this data to characterize attenuation, scattering microstructure and microvascular function. Invasive reference measurements (microvascular density, cell viability and necrosis, IFP) will also be associated with corresponding 3D mappings and follow-up will be completed by survival curves (Mantel-Cox). Multiparametric classification based on combination of sets of imaging parameters will be tested in terms of sensitivity to changes occurring in tumors as demonstrated by reference data and then applied to longitudinal follow-up.
Results will help to better understand and predict effects of this novel therapeutic approach and contribute to development of ultrasonic techniques for in vivo monitoring.
Dates / Durée du stage
6 mois, printemps 2015
Contact:
S Lori Bridal, Laboratoire d’Imagerie Biomédicale,
Pour candidature merci d’envoyer votre CV en décrivant vos motivations, svp. Capacités d’expérimentation, traitement d’image, acoustiques, biologie et communication/travail en équipe souhaitées.
Lieu de stage:
15 rue de l’école de médecine 75006 Paris CEDEX 6
Rémunération:
gratifications légales de stage
